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Anti-Nephrin Antibody (A306577)

Western Blot - Anti-Nephrin Antibody (A306577) - Antibodies.com
$470
Product Datasheet

Rabbit polyclonal antibody to Nephrin for WB.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Nephrin Antibody
Description
Rabbit polyclonal antibody to Nephrin.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1019-1241 of human NPHS1 (NP_004637.1).
Sequence
LGDSGLADKGTQLPITTPGLHQPSGEPEDQLPTEPPSGPSGLPLLPVLFALGGLLLLSNASCVGGVLWQRRLRRLAEGISEKTEAGSEEDRVRNEYEESQWTGERDTQSSTVSTTEAEPYYRSLRDFSPQLPPTQEEVSYSRGFTGEDEDMAFPGHLYDEVERTYPPSGAWGPLYDEVQMGPWDLHWPEDTYQDPRGIYDQVAGDLDTLEPDSLPFELRGHLV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
200 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CNF, Nephrosis 1 congenital Finnish type, Nephrosis 1, congenital, Finnish type (nephrin), NPHN, NPHN_HUMAN, NPHS 1, Nphs1, Renal glomerulus specific cell adhesion receptor, Renal glomerulus-specific cell adhesion receptor
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Nephrin Antibody (A306577) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Nephrin Antibody (A306577)
Western blot analysis of extracts from wild type(WT) and NPHS1 knockdown (KD) A-549 cells, using Anti-Nephrin Antibody (A306577) at 1:800 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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