Rabbit monoclonal [ARC57532] antibody to Neurogranin.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein of human Neurogranin (NRGN).
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC57532
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Molecular Weight
15 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Calmodulin binding protein, Hng, NEUG(55-78), NEUG_HUMAN, Neurogranin (protein kinase C substrate), Ng, NRGN, Protein kinase C substrate, Protein kinase C substrate RC3, RC3
Figure 1: Western Blot - Anti-Neurogranin Antibody [ARC57532] (A309948)
Western blot analysis of various lysates, using Anti-Neurogranin Antibody [ARC57532] (A309948) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Enhanced Kit (RM00021). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-Neurogranin Antibody [ARC57532] (A309948) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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