Anti-NF-kB p65 Antibody [ARC51088] (A306572)

Name: Anti-NF-kB p65 Antibody [ARC51088]
Brand: Antibodies.com
SKU: A306572
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51088] antibody to NF-kB p65 for WB, IHC, ICC/IF, IP and ChIP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-NF-kB p65 Antibody [ARC51088]

Description

Rabbit monoclonal [ARC51088] antibody to NF-kB p65.

Applications

WB, IHC, ICC/IF, IP, ChIP

Dilutions

WB: 1:2,000-1:10,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200, IP: 1:50-1:200, ChIP: 1:50-1:200

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-kB p65/RelA (NP_068810.3).

Sequence

LGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51088

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Avian reticuloendotheliosis viral (v rel) oncogene homolog A, MGC131774, NF kappa B p65delta3, NFKB3, Nuclear Factor NF Kappa B p65 Subunit, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, OTTHUMP00000233473, OTTHUMP00000233474, OTTHUMP00000233475, OTTHUMP00000233476, OTTHUMP00000233900, p65, p65 NF kappaB, p65 NFkB, relA, TF65_HUMAN, Transcription factor p65, V rel avian reticuloendotheliosis viral oncogene homolog A, v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)), v rel reticuloendotheliosis viral oncogene homolog A (avian), V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Western Blot - Anti-NF-kB p65 Antibody [ARC51088] (A306572)

Western blot analysis of extracts of various cell lines, using Anti-NF-kB p65 Antibody [ARC51088] (A306572) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-NF-kB p65 Antibody [ARC51088] (A306572)

Western blot analysis of extracts of various cell lines, using Anti-NF-kB p65 Antibody [ARC51088] (A306572) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Western Blot - Anti-NF-kB p65 Antibody [ARC51088] (A306572)

Western blot analysis of extracts from wild type(WT) and NF-kB p65/RelA knockout (KO) HeLa cells, using Anti-NF-kB p65 Antibody [ARC51088] (A306572) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 4: Immunohistochemistry - Anti-NF-kB p65 Antibody [ARC51088] (A306572)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-NF-kB p65 Antibody [ARC51088] (A306572) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.