NF-kB p65 (phospho Ser311) Cell Based ELISA Kit (A103769)

NF-kB p65 (phospho Ser311) Cell Based ELISA Kit

Cell-based (qualitative)

This NF-kB p65 (phospho Ser311) Cell Based ELISA Kit allows for the detection of NF-kB p65 (phospho Ser311) and the effects that certain stimulation conditions have on NF-kB p65 (phospho Ser311) expression in different cell lines. Qualitative determination of NF-kB p65 (phospho Ser311) concentration is achieved by an indirect ELISA format. In essence, NF-kB p65 (phospho Ser311) is captured by Anti-NF-kB p65 (phospho Ser311) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-NF-kB p65 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Microplate

> 5000 Cells

Human, Mouse

Store at + 4°C. Stable for 6 months.

Avian reticuloendotheliosis viral (v rel) oncogene homolog A, MGC131774, NF kappa B p65delta3, NFKB3, Nuclear Factor NF Kappa B p65 Subunit, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B cells 3, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, OTTHUMP00000233473, OTTHUMP00000233474, OTTHUMP00000233475, OTTHUMP00000233476, OTTHUMP00000233900, p65, p65 NF kappaB, p65 NFkB, relA, TF65_HUMAN, Transcription factor p65, V rel avian reticuloendotheliosis viral oncogene homolog A, v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)), v rel reticuloendotheliosis viral oncogene homolog A (avian), V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65