This antibody recognises chondroitin sulfate proteoglycan NG2 also known as chondroitin sulfate proteoglycan 4 (CSPG4) and melanoma chondroitin sulfate proteoglycan. It was first characterized in rat as a high-molecular-weight type 1 membrane proteoglycan (Stallcup 1981). CSPG4 is involved in cell attachment and migration. It regulates spreading of melanoma cells by recruiting and activating a signalling cascade through CDC42, ACK1 and p130cas. It enhances FAK and ERK activation.

Applications

Dilutions

WB: 1:500 - 1:2,000

Reactivity

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human chondroitin sulphate proteoglycan NG2.

Sequence

LEVPQALGRSSLSQQQLRVVSDREEPEAAYRLIQGPQYGHLLVGGRPTSAFSQFQIDQGEVVFAFTNFSSSHDHFRVLALARGVNASAVVNVTVRALLHVWAGGPWPQGATLRLDPTVLDAGELANRTGSVPRFRLLEGPRHGRVVRVPRARTEPGGSQLVEQFTQQDLEDGRLGLEVGRPEGRAPGPAGDSLTLELWAQGVPPAVASLDFATEPYNAARPYSVALLSVPEAARTEAGKPESSTPTGEPGPMASSPEPAVA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Concentration

Lot Specific

Molecular Weight

Approximately 320 kDa in HeLa and A375 cell lysates under reducing conditions

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 0.05% ProClin 300 and 50% Glycerol.

Storage

Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

General Notes

Rabbit anti chondroitin sulphate proteoglycan NG2 antibody recognizes chondroitin sulfate proteoglycan NG2 also known as chondroitin sulfate proteoglycan 4 (CSPG4) and melanoma chondroitin sulfate proteoglycan. It was first characterized in rat as a high-molecular-weight type 1 membrane proteoglycan (Stallcup 1981). CSPG4 is involved in cell attachment and migration (Karram et al. 2005). It regulates spreading of melanoma cells by recruiting and activating a signalling cascade through CDC42, ACK1 and p130cas (Eisenmann et al. 1999). It enhances FAK and ERK activation (Yang et al. 2004).

Synonyms

4732461B14Rik, AN2, AN2 proteoglycan, Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan 4 (melanoma-associated), Chondroitin sulfate proteoglycan NG2, Cspg4, Cspg4 chondroitin sulfate proteoglycan 4, CSPG4_HUMAN, HMW-MAA, HSN tumor-specific antigen, Kiaa4232, MCSP, MCSPG, MEL-CSPG, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan, MELCSPG, MSK16

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Publishing research using Anti-NG2 Antibody (A283726)? Please let us know so that we can list the citation on this page.