Anti-Lipocalin-2 / NGAL Antibody [D02-7D4] (Biotin) (A280197)

Anti-Lipocalin-2 / NGAL Antibody [D02-7D4] (Biotin)

Mouse monoclonal [D02-7D4] antibody to Lipocalin-2 / NGAL (Biotin).

This antibody recognises Neutrophil Gelatinase-Associated Lipocalin (NGAL) also known as lipocalin-2, 24 kDa alpha-2-microglobin-related subunit of MMP-9 and siderocalin. NGAL is a 198 amino acid 25 kDa secreted protein which possesses a lipid binding domain which enables it to bind small hydrophobic ligand molecules such as bacterial siderophores (Bauvois & Susin 2018, Abella et al. 2015). As well as the 25 kDa form, NGAL can exist as a 46 kDa homodimer and a 130 kDa heterodimer with MMP-9. The interaction with MMP-9 occurs in the extracellular space upon secretion of both proteins. It is through this interaction that MMP-9 proteolytic activity can be inhibited. NGAL is expressed in various cell types including adipocytes, hepatocytes, pneumocytes, splenocytes, mesangial and microglial cells. NGAL has also been observed circulating at low levels in urine and blood of healthy subjects (40-109 ng/ml). However it is overexpressed during various inflammatory, metabolic, neurologic and cancer pathologies. The higher levels of NGAL expression in these diseases have been correlated with disease severity and poor prognosis (Bauvois & Susin 2018, Chakraborty et al. 2012). The biotinylated Mouse anti Human NGAL antibody, clone D02-7D4 (MCA6199B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human NGAL antibody, clone D11-6D10 (MCA6198GA) as the capture antibody.

ELISA: 1 µg/ml

Human

E. coli derived recombinant human NGAL (Gln21-Gly198).

Mouse

Monoclonal

D02-7D4

IgG1

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human NGAL antibody, clone D02-7D4, recognizes Neutrophil Gelatinase-Associated Lipocalin (NGAL) also known as lipocalin-2, 24 kDa alpha-2-microglobin-related subunit of MMP-9 and siderocalin. NGAL is a 198 amino acid 25 kDa secreted protein which possesses a lipid binding domain which enables it to bind small hydrophobic ligand molecules such as bacterial siderophores (Bauvois & Susin 2018, Abella et al. 2015). As well as the 25 kDa form, NGAL can exist as a 46 kDa homodimer and a 130 kDa heterodimer with MMP-9. The interaction with MMP-9 occurs in the extracellular space upon secretion of both proteins. It is through this interaction that MMP-9 proteolytic activity can be inhibited (Chakraborty et al. 2012). NGAL is expressed in various cell types including adipocytes, hepatocytes, pneumocytes, splenocytes, mesangial and microglial cells. NGAL has also been observed circulating at low levels in urine and blood of healthy subjects (40-109 ng/ml) . However it is overexpressed during various inflammatory, metabolic, neurologic and cancer pathologies. The higher levels of NGAL expression in these diseases have been correlated with disease severity and poor prognosis (Bauvois & Susin 2018, Chakraborty et al. 2012). The biotinylated Mouse anti Human NGAL antibody, clone D02-7D4 (MCA6199B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human NGAL antibody, clone D11-6D10 (MCA6198GA) as the capture antibody.

24p3, 25 kDa alpha 2 microglobulin related subunit of MMP9, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Alpha 2 microglobulin related protein, HGNC:6526, HNL, Lcn 2, Lcn2, Lipocalin 2, Lipocalin-2, Migration stimulating factor inhibitor, MSFI, Neutrophil gelatinase associated lipocalin, Neutrophil gelatinase associated lipocalin precursor, Neutrophil gelatinase-associated lipocalin, NGAL, NGAL_HUMAN, Oncogene 24p3, Oncogenic lipocalin 24p3, p25, Siderocalin, siderocalin LCN2, SV40 induced 24P3 protein, Uterocalin

This product is for research use only. It is not intended for diagnostic or therapeutic use.