Anti-NgR3 Antibody [ARC2933] (A308171)

Name: Anti-NgR3 Antibody [ARC2933]
Brand: Antibodies.com
SKU: A308171

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This product is discontinued

Anti-NgR3 Antibody [ARC2933] (A308171) has been discontinued and is no longer available.

View all Anti-NgR3 Antibodies.

Specifications

Name

Anti-NgR3 Antibody [ARC2933]

Description

Rabbit monoclonal [ARC2933] antibody to NgR3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 342-441 of human NgR3 (Q86UN2).

Sequence

PHGPRPGHRKPGKNCTNPRNRNQISKAGAGKQAPELPDYAPDYQHKFSFDIMPTARPKRKGKCARRTPIRAPSGVQQASSASSLGASLLAWTLGLAVTLR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2933

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

49 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp547J144, NgR 3, NGRH 2, NGRH2, NGRL2, Nogo 66 receptor homolog 2, Nogo 66 receptor related protein 3, Nogo receptor like 2, Nogo receptor-like 2, Nogo-66 receptor homolog 2, Nogo-66 receptor-related protein 3, R4RL1_HUMAN, Reticulon 4 receptor like 1, Reticulon-4 receptor-like 1, RTN4RL1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Western Blot - Anti-NgR3 Antibody [ARC2933] (A308171)

Western blot analysis of extracts of various cell lines, using Anti-NgR3 Antibody [ARC2933] (A308171) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunohistochemistry - Anti-NgR3 Antibody [ARC2933] (A308171)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-NgR3 Antibody [ARC2933] (A308171) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-NgR3 Antibody [ARC2933] (A308171)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-NgR3 Antibody [ARC2933] (A308171) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-NgR3 Antibody [ARC2933] (A308171)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-NgR3 Antibody [ARC2933] (A308171) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.