Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528)

Name: Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody
Brand: Antibodies.com
SKU: A13528
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Nicotinic Acetylcholine Receptor alpha 7 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody

Description

Rabbit polyclonal antibody to Nicotinic Acetylcholine Receptor alpha 7.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 23-230 of human CHRNA7 (NP_001177384.1).

Sequence

ASPPSTPPWDPGHIPGASVRPAPGPVSLQGEFQRKLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNVSEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQHCKLKFGSWSYGGWSLDLQMQEADISGYIPNGEWDLVGI

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

53 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

7nAChR, a7 nicotinic acetylcholine receptor, acetylcholine receptor, neuronal nicotinic, alpha-7 subunit, ACHA7_HUMAN, AChR, Acra7, alpha 7 neuronal nicotinic acetylcholine receptor, alpha-7 nicotinic cholinergic receptor subunit, alpha7, cholinergic receptor, neuronal nicotinic, alpha polypeptide 7, cholinergic receptor, nicotinic, alpha 7, cholinergic receptor, nicotinic, alpha 7 (neuronal), cholinergic receptor, nicotinic, alpha polypeptide 7, CHRNA7, CHRNA7-2, NACHRA7, neuronal acetylcholine receptor protein, alpha-7 chain, Neuronal acetylcholine receptor subunit alpha-7

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528)

Western blot analysis of extracts of various cell lines, using Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528)

Immunofluorescence analysis of L929 cells using Anti-Nicotinic Acetylcholine Receptor alpha 7 Antibody (A13528) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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