Anti-Niemann Pick C1 Antibody [ARC2713] (A307458)

Name: Anti-Niemann Pick C1 Antibody [ARC2713]
Brand: Antibodies.com
SKU: A307458
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC2713] antibody to Niemann Pick C1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Niemann Pick C1 Antibody [ARC2713]

Description

Rabbit monoclonal [ARC2713] antibody to Niemann Pick C1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1179-1278 of human Niemann Pick C1 (O15118).

Sequence

MKGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKSCATEERYKGTERERLLNF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2713

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

180 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Niemann Pick C1 protein precursor, Niemann Pick disease, type C1, Niemann-Pick C1 protein, NPC, NPC1, NPC1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Niemann Pick C1 Antibody [ARC2713] (A307458)

Western blot analysis of extracts of Mouse liver, using Anti-Niemann Pick C1 Antibody [ARC2713] (A307458) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Niemann Pick C1 Antibody [ARC2713] (A307458)

Western blot analysis of extracts of various cell lines, using Anti-Niemann Pick C1 Antibody [ARC2713] (A307458) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunohistochemistry - Anti-Niemann Pick C1 Antibody [ARC2713] (A307458)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Niemann Pick C1 Antibody [ARC2713] (A307458) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Niemann Pick C1 Antibody [ARC2713] (A307458)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Niemann Pick C1 Antibody [ARC2713] (A307458) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.