Anti-NMDAR2B Antibody [S59] (A304796)

Name: Anti-NMDAR2B Antibody [S59]
Brand: Antibodies.com
SKU: A304796
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S59] antibody to NMDAR2B for WB, IHC, ICC/IF, IP and Antibody Microarray.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-NMDAR2B Antibody [S59]

Description

Mouse monoclonal [S59] antibody to NMDAR2B.

Applications

WB, IHC, ICC/IF, IP, Antibody Microarray

Dilutions

WB: 1:1,000, IHC: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with NR2A.

Immunogen

Fusion protein corresponding to the extracellular N-terminus, amino acids 20-271, of rat NR2B protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

S59

Isotype

IgG2b

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~166 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AW490526, EIEE27, Glutamate Receptor Ionotropic N Methyl D Aspartate 2B, Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B, Glutamate receptor ionotropic NMDA2B, Glutamate receptor subunit epsilon 2, Glutamate receptor, ionotropic, NMDA2B (epsilon 2), Glutamate [NMDA] receptor subunit epsilon 2, Glutamate [NMDA] receptor subunit epsilon-2, GRIN 2B, GRIN2B, hNR 3, hNR3, MGC142178, MGC142180, MRD6, N methyl D asparate receptor channel subunit epsilon 2, N methyl D aspartate receptor subtype 2B, N methyl D aspartate receptor subunit 2B, N methyl D aspartate receptor subunit 3, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NMDA NR2B, NMDA R2B, NMDE2, NMDE2_HUMAN, NME2, NR2B, NR3

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-NMDAR2B Antibody [S59] (A304796)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of GluN2B/NR2B protein using Anti-NMDAR2B Antibody [S59] (A304796) at 1:250.

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Figure 2: Western Blot - Anti-NMDAR2B Antibody [S59] (A304796)

Western blot analysis of rat brain membrane lysate showing detection of GluN2B/NR2B protein using Anti-NMDAR2B Antibody [S59] (A304796) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 3: Immunohistochemistry - Anti-NMDAR2B Antibody [S59] (A304796)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-NMDAR2B Antibody [S59] (A304796) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Filaggrin-like staining, and dermal staining.

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Figure 4: Immunofluorescence - Anti-NMDAR2B Antibody [S59] (A304796)

Immunocytochemistry/Immunofluorescence analysis of human HaCaT cells, fixed in cold 100% methanol for 10 minutes at -20°C, using Anti-NMDAR2B Antibody [S59] (A304796), at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Everything positive.