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Anti-Notch 1 (cleaved Val1754) Antibody (A93620)

This product is identical to Sigma (SAB4502019). Discover more for less.

Western Blot - Anti-Notch 1 (cleaved Val1754) Antibody (L0119) - Antibodies.com Western Blot - Anti-Notch 1 (cleaved Val1754) Antibody (L0119) - Antibodies.com Western Blot - Anti-Notch 1 (cleaved Val1754) Antibody (L0119) - Antibodies.com Immunofluorescence - Anti-Notch 1 (cleaved Val1754) Antibody (L0119) - Antibodies.com Immunofluorescence - Anti-Notch 1 (cleaved Val1754) Antibody (L0119) - Antibodies.com
$185
Product Datasheet

Rabbit polyclonal antibody to Notch 1 (cleaved Val1754) for WB, IHC and ELISA.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Notch 1 (cleaved Val1754) Antibody
Description
Rabbit polyclonal antibody to Notch 1 (cleaved Val1754).
Specificity
This antibody detects endogenous levels of fragment of activated Notch 1 resulting from cleavage adjacent to Val1754.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Notch 1 (amino acids 1735-1784).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
110kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
hN1, Neurogenic locus Notch homolog protein 1, NICD, NOTC1_HUMAN, Notch 1, Notch 1 intracellular domain, Notch homolog 1 translocation associated, notch1, TAN1, Translocation associated Notch protein TAN1, Translocation-associated notch protein TAN-1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Notch 1 (cleaved Val1754) Antibody (L0119) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Notch 1 (cleaved Val1754) Antibody (A93620)
Western blot analysis of lysates from NIH/3T3 cells, treated with Etoposide 25uM 60' using Anti-Notch 1 (cleaved Val1754) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Western Blot - Anti-Notch 1 (cleaved Val1754) Antibody (L0119) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Notch 1 (cleaved Val1754) Antibody (A93620)
Western blot analysis of various cells using Anti-Notch 1 (cleaved Val1754) Antibody.
Western Blot - Anti-Notch 1 (cleaved Val1754) Antibody (L0119) - Antibodies.comEnlarge Image
Figure 3: Western Blot - Anti-Notch 1 (cleaved Val1754) Antibody (A93620)
Western blot analysis of KB cells using Anti-Notch 1 (cleaved Val1754) Antibody.
Immunofluorescence - Anti-Notch 1 (cleaved Val1754) Antibody (L0119) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-Notch 1 (cleaved Val1754) Antibody (A93620)
Immunofluorescence analysis of human lung cancer tissue using Anti-Notch 1 (cleaved Val1754) Antibody (red) at 1:200 (4°C overnight). Cy3 labelled secondary antibody was used at 1:300 (RT 50min). Panel A: Target. Panel B: DAPI. Panel C: Merge.
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