Anti-Notch1 Antibody [S253-32] (A304795)

Name: Anti-Notch1 Antibody [S253-32]
Brand: Antibodies.com
SKU: A304795
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S253-32] antibody to Notch1 for WB and ICC/IF.

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$40 to United States

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5-8 Business Days

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Neuroepithelial Marker

Highlighted products for Anti-Notch1 Antibody

A86356

8 Citations

Anti-Notch1 Antibody [mN1A]

Mouse monoclonal [mN1A] antibody to Notch1 for Flow Cytometry, IP, WB, IHC-Fr and ICC.

A86356

8 Citations

Anti-Notch1 Antibody [mN1A]

Mouse monoclonal [mN1A] antibody to Notch1 for Flow Cytometry, IP, WB, IHC-Fr and ICC.

Specifications

Name

Anti-Notch1 Antibody [S253-32]

Description

Mouse monoclonal [S253-32] antibody to Notch1.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with Notch 2 or Notch 3.

Immunogen

Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1.

Host

Mouse

Clonality

Monoclonal

Clone ID

S253-32

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

>270 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

9930111A19Rik, AOS5, AOVD1, hN1, Lin-12, LIN12, Mis6, Motch A, mT14, Neurogenic locus notch homolog protein 1, Neurogenic locus notch protein homolog, NICD, NOTC1_HUMAN, NOTCH, Notch 1, Notch 1 intracellular domain, Notch gene homolog 1 (Drosophila), Notch homolog 1, translocation-associated (Drosophila), NOTCH, Drosophila, homolog of, 1, TAN1, Translocation associated notch homolog, Translocation associated notch protein TAN 1, Translocation-associated notch protein TAN-1, xotch

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-Notch1 Antibody [S253-32] (A304795)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-Notch1 Antibody [S253-32] (A304795), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Notch1 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-Notch1 Antibody [S253-32] (A304795)

Western blot analysis of rat brain membrane showing detection of ~270 kDa Notch1 protein using Anti-Notch1 Antibody [S253-32] (A304795) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat brain membrane (10 µg).. Load: 10 µg. Block: 5% milk. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at room temperature. Color Development: TMB solution for 10 min at room temperature. Predicted/Observed Size: ~270 kDa.

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Figure 3: Immunofluorescence - Anti-Notch1 Antibody [S253-32] (A304795)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Notch1 Antibody [S253-32] (A304795), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cell Membrane, Nucleus. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Notch1 Antibody. (D) Composite.

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