Anti-NPAS4 Antibody [S408-79] (A305032)

Name: Anti-NPAS4 Antibody [S408-79]
Brand: Antibodies.com
SKU: A305032
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S408-79] antibody to NPAS4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-NPAS4 Antibody [S408-79]

Description

Mouse monoclonal [S408-79] antibody to NPAS4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 597-802 (C-terminus) of rat Npas4

Host

Mouse

Clonality

Monoclonal

Clone ID

S408-79

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

bHLH PAS type transcription factor NXF, bHLHe79, Class E basic helix-loop-helix protein 79, HLH-PAS transcription factor NXF, Le PAS, limbic enhanced PAS protein, neuronal PAS domain protein 4, Neuronal PAS domain-containing protein 4, Neuronal PAS4, NPAS4_HUMAN, NXF, PAS domain-containing protein 10, PASD10

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-NPAS4 Antibody [S408-79] (A305032)

Western blot analysis of rat brain showing detection of ~90 kDa NPAS4 protein using Anti-NPAS4 Antibody [S408-79] (A305032) at 1:1,000 for 16 hours at 4°C. Lane 1: MW Ladder. Lane 2: rat Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at room temperature. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.

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Figure 2: Immunofluorescence - Anti-NPAS4 Antibody [S408-79] (A305032)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-NPAS4 Antibody [S408-79] (A305032), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.

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