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Anti-NPHS2 Antibody [ARC1907] (A305404)

Western Blot - Anti-NPHS2 Antibody [ARC1907] (A305404) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC1907] antibody to NPHS2 for WB.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-NPHS2 Antibody [ARC1907]
Description
Rabbit monoclonal [ARC1907] antibody to NPHS2.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 284-383 of human NPHS2 (Q9NP85).
Sequence
AQRQAKVRMIAAEAEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPSTVVLPLPFDLLNCLSSPSNRTQGSLPFPSPSKPVEPLNPKKKDSPML
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1907
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
35 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
nephrosis 2, idiopathic, steroid resistant, Nephrosis 2, idiopathic, steroid resistant (podocin), NPHS2 gene, PDCN, Podocin, PODO_HUMAN, SRN1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-NPHS2 Antibody [ARC1907] (A305404) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-NPHS2 Antibody [ARC1907] (A305404)
Western blot analysis of extracts of various cell lines, using Anti-NPHS2 Antibody [ARC1907] (A305404) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
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