Anti-NR1D1 Antibody (A308755)

Name: Anti-NR1D1 Antibody
Brand: Antibodies.com
SKU: A308755
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to NR1D1 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-NR1D1 Antibody

Description

Rabbit polyclonal antibody to NR1D1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 280-360 of human Rev-Erba/NR1D1 (NP_068370.1).

Sequence

SPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQRHNEALNGLRQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

78 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EAR-1, EAR1, ERBA-related 1, hRev, NR1D1_HUMAN, Nuclear receptor Rev ErbA alpha, Nuclear receptor subfamily 1 group D member 1, Rev erbAalpha, Rev erbalpha, Rev-erbA-alpha, Rev-ErbAalpha, Reverba, THRA1, THRAL, Thyroid hormone receptor, alpha like, Thyroid hormone receptor, alpha-1- like, V-erbA-related protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-NR1D1 Antibody (A308755)

Western blot analysis of extracts of HepG2 cells, using Anti-NR1D1 Antibody (A308755) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-NR1D1 Antibody (A308755)

Western blot analysis of extracts of Rat lung, using Anti-NR1D1 Antibody (A308755) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-NR1D1 Antibody (A308755)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-NR1D1 Antibody (A308755) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-NR1D1 Antibody (A308755)

Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-NR1D1 Antibody (A308755) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.