Anti-NSDHL Antibody (A92660)

Name: Anti-NSDHL Antibody
Brand: Antibodies.com
SKU: A92660
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to NSDHL for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-NSDHL Antibody

Description

Rabbit polyclonal antibody to NSDHL.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NSDHL (NP_057006.1).

Sequence

MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

40 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

decarboxylating, H105E3, H105e3 protein, NAD(P) dependent steroid dehydrogenase like, NSDHL_HUMAN, Protein H105e3, SDR31E1, Short chain dehydrogenase/reductase family 31E member 1, Sterol 4 alpha carboxylate 3 dehydrogenase decarboxylating, Sterol-4-alpha-carboxylate 3-dehydrogenase, XAP104

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-NSDHL Antibody (A92660)

Western blot analysis of extracts from wild type(WT) and NSDHL knockout (KO) 293T(KO) cells, using Anti-NSDHL Antibody (A92660) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-NSDHL Antibody (A92660)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-NSDHL Antibody (A92660) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-NSDHL Antibody (A92660)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-NSDHL Antibody (A92660) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.