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Anti-nSMase-3 Antibody (A87716)

Western Blot - Anti-nSMase-3 Antibody (A87716) - Antibodies.com Immunofluorescence - Anti-nSMase-3 Antibody (A87716) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to nSMase-3 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-nSMase-3 Antibody
Description
Rabbit polyclonal antibody to nSMase-3.
Applications
WB, ICC/IF
Dilutions
WB: 1:200-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 600-800 of human SMPD4 (NP_060421.2).
Sequence
ITQAKHTAKSISDQCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSVRKTDEYLEKALEYLRQIFRLSEAQLRQFTLALGTTQDENGKKQLPDCIVGEDGLILTPLGRYQIINGLRRFEIEYQGDPELQPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCSRDDFLGSFCRYHLTEPGLASRHLLSPVGRRQVA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
100 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
EC 3.1.4.12, EC=3.1.4.123, FLJ20297, FLJ20756, KIAA1418, NET 13, NET13, Neutral sphingomyelinase 3, Neutral sphingomyelinase III, NSMA3_HUMAN, nSMase 3, nSMase3, OTTHUMP00000162391, OTTHUMP00000190862, SKNY protein, SMPD4, Sphingomyelin phosphodiesterase 4, Sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase 3)
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-nSMase-3 Antibody (A87716) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-nSMase-3 Antibody (A87716)
Western blot analysis of extracts of A-549 cells, using Anti-nSMase-3 Antibody (A87716) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 15s.
Immunofluorescence - Anti-nSMase-3 Antibody (A87716) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-nSMase-3 Antibody (A87716)
Immunofluorescence analysis of L929 cells using Anti-nSMase-3 Antibody (A87716) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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