Anti-NUDT19 Antibody [ARC2938] (A306248)

Name: Anti-NUDT19 Antibody [ARC2938]
Brand: Antibodies.com
SKU: A306248

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This product is discontinued

Anti-NUDT19 Antibody [ARC2938] (A306248) has been discontinued and is no longer available.

View all Anti-NUDT19 Antibodies.

Specifications

Name

Anti-NUDT19 Antibody [ARC2938]

Description

Rabbit monoclonal [ARC2938] antibody to NUDT19.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 60-190 of human NUDT19 (A8MXV4).

Sequence

HVFSGGVLDAADRSADWLGLFAPHHGPPRFGLGPAPFSRTAFPSLPDTDDHKTDNTGTLPEDVAFRICAVREAFEEAGVLLLRPRTSPPGPAPGPGLALEPPPGLASWRDRVRQDPRHFLRLCAHLDCTPD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2938

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

45 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

mitochondrial, Nucleoside diphosphate-linked moiety X motif 19, nucleoside diphosphate-linked moiety X motif 19, mitochondrial, NUD19_HUMAN, nudix (nucleoside diphosphate linked moiety X)-type motif 19, Nudix motif 19, RP2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-NUDT19 Antibody [ARC2938] (A306248)

Western blot analysis of extracts of various cell lines, using Anti-NUDT19 Antibody [ARC2938] (A306248) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-NUDT19 Antibody [ARC2938] (A306248)

Western blot analysis of extracts of Rat heart, using Anti-NUDT19 Antibody [ARC2938] (A306248) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-NUDT19 Antibody [ARC2938] (A306248)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-NUDT19 Antibody [ARC2938] (A306248) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-NUDT19 Antibody [ARC2938] (A306248)

Immunofluorescence analysis of K-562 cells using Anti-NUDT19 Antibody [ARC2938] (A306248) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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