Anti-NuMA Antibody (A12645)

Name: Anti-NuMA Antibody
Brand: Antibodies.com
SKU: A12645
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to NuMA for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Centrosome Marker

Specifications

Name

Anti-NuMA Antibody

Description

Rabbit polyclonal antibody to NuMA.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:200-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human NUMA1 (NP_006176.2).

Sequence

MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

238 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Centrophilin stabilizes mitotic spindle in mitotic cells, NMP 22, Nuclear matrix protein 22, Nuclear mitotic apparatus protein, Nuclear mitotic apparatus protein 1, NUMA 1, NuMA protein, NUMA1, NUMA1_HUMAN, SP H antigen, SP-H antigen, Structural nuclear protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-NuMA Antibody (A12645)

Western blot analysis of extracts of various cell lines, using Anti-NuMA Antibody (A12645) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-NuMA Antibody (A12645)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-NuMA Antibody (A12645) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-NuMA Antibody (A12645)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-NuMA Antibody (A12645) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-NuMA Antibody (A12645)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-NuMA Antibody (A12645) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.