One-step Rapid Dewaxing Solution (A293490)

One-step Rapid Dewaxing Solution

This one step rapid dewaxing solution is a rapid reagent for paraffin slides pretreatment. There is no need to do the traditional three times of xylene dewaxing, three to five times of gradient ethanol hydration, but only one Dewaxing solution. The operation time is effectively shortened, the cumbersome operation steps are simplified so the variables in operation are reduced, and the stability is improved. The Dewaxing solution applies new environmental protection technology is not only reduces the harm to the body, but also cause no pollution to the environment.

TUNEL/IHC

Dewaxing solution (10×) is a 10× concentrated solution. Dilute with ddH20 to 1 ×Dewaxing working solution before use.

Experimental operation steps: 1. Dilute the dewaxing solution (10×) with ddH2O at the ratio of 1:9. Note: Flocculent sediment will be generated during dilution. Do not blow it with any pipette. Please bake the diluted solution at 60°C in an oven for 30 min, then stirred or blown to mix evenly, and finally the solution present as milky white suspension. 2. Dewax the slides with 1× Dewaxing solution. Rewarm the dewaxing solution at 60°C for 30 min, immerse the slides into the Dewaxing solution and incubate at 60°C for 30 min. Note: Low temperature may affect the effect of dewaxing. Please rewarm the dewaxing solution at 60°C for 30 min before the dewaxing. If the paraffin slides are thick, you can dewax the slides for 50 min. 3. Wash the slides with tap water for 5 min until froth free. Note: Don’t wash the tissue directly, avoid tissue damage. 4. Absorbs the moisture around the tissue with filter paper for the next step. Or immerse the slides in PBS for use. 5. For IHC experiment, follow the antigen repair step. For TUNEL or HE staining experiment, please follow the staining kit steps.