This antibody recognises the human orexin A subunit of orexin, also known as hypocretin. Orexin is cleaved into Orexin A, also known as hypocretin-1, a 32 amino acid excitatory neuropeptide linked to alertness and wakefulness in humans. Orexin A binds to both orexin receptors 1 and 2 with similar affinity whilst orexin B corresponding to the C terminal region of the holopeptide binds only to the orexin receptor 2.Mutations in the orexin gene can lead to the development of Narcolepsy 1 (NRCLP1), a condition characterized by sleep fragmentation and daytime drowziness, abnormal REM sleep and sleep paralysis along with other sleep related symptoms. Sheep anti Human orexin A antibody is a useful reagent for immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Synthetic peptide sequence PLPDCCRQKTCSC corresponding to amino acids 2-14 of human orexin A conjugated to Keyhole Limpet Haemocyanin.
Sequence
PLPDCCRQKTCSC
Host
Sheep
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
5 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Sheep anti Human orexin A antibody recognizes the human orexin A subunit of orexin, also known as hypocretin. Orexin is cleaved into Orexin A, also known as hypocretin-1, a 32 amino acid excitatory neuropeptide linked to alertness and wakefulness in humans. Orexin A binds to both orexin receptors 1 and 2 with similar affinity whilst orexin B corresponding to the C terminal region of the holopeptide binds only to the orexin receptor 2.Mutations in the orexin gene can lead to the development of Narcolepsy 1 (NRCLP1), a condition characterized by sleep fragmentation and daytime drowziness, abnormal REM sleep and sleep paralysis along with other sleep related symptoms (Peyron et al. 2000).Sheep anti Human orexin A antibody is a useful reagent for immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
