Anti-Osteocalcin Antibody (A88004)

Name: Anti-Osteocalcin Antibody
Brand: Antibodies.com
SKU: A88004

Enlarge Image

This product is discontinued

Anti-Osteocalcin Antibody (A88004) has been discontinued and is no longer available.

View all Anti-Osteocalcin Antibodies.

Specifications

Name

Anti-Osteocalcin Antibody

Description

Rabbit polyclonal antibody to Osteocalcin.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-60 of human Osteocalcin (BGLAP) (NP_954642.1).

Sequence

MRALTLLALLALAALCIAGQAGAKPSGAESSKGAAFVSKQEGSEVVKRPRRYLYQWLGAP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

11 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BGLAP, BGP, Bone gamma carboxyglutamate (gla) protein, Bone gamma carboxyglutamate gla protein osteocalcin, Bone gamma carboxyglutamate protein, Bone Gla protein, Gamma carboxyglutamic acid containing protein, Gamma-carboxyglutamic acid-containing protein, OC, OCN, OSTCN_HUMAN, OTTHUMP00000016586, PMF1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

Enlarge Image

Figure 1: Western Blot - Anti-Osteocalcin Antibody (A88004)

Western blot analysis of Rat lung, using Anti-Osteocalcin Antibody (A88004) at 1:1,500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Enlarge Image

Figure 2: Immunofluorescence - Anti-Osteocalcin Antibody (A88004)

Immunofluorescence analysis of MG-63 cells using Anti-Osteocalcin Antibody (A88004) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 3: Immunofluorescence - Anti-Osteocalcin Antibody (A88004)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Osteocalcin Antibody (A88004) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

Enlarge Image

Figure 4: Immunofluorescence - Anti-Osteocalcin Antibody (A88004)

Immunofluorescence analysis of U2OS cells using Anti-Osteocalcin Antibody (A88004) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

Publishing research using Anti-Osteocalcin Antibody (A88004)? Please let us know so that we can list the citation on this page.