Anti-OTUD6B Antibody (A89659)

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This product is discontinued

Anti-OTUD6B Antibody (A89659) has been discontinued and is no longer available.

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Specifications

Name

Anti-OTUD6B Antibody

Description

Rabbit polyclonal antibody to OTUD6B.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 31-323 of human OTUD6B (NP_057107.3).

Sequence

MEAVLTEELDEEEQLLRRHRKEKKELQAKIQGMKNAVPKNDKKRRKQLTEDVAKLEKEMEQKHREELEQLKLTTKENKIDSVAVNISNLVLENQPPRISKAQKRREKKAALEKEREERIAEAEIENLTGARHMESEKLAQILAARQLEIKQIPSDGHCMYKAIEDQLKEKDCALTVVALRSQTAEYMQSHVEDFLPFLTNPNTGDMYTPEEFQKYCEDIVNTAAWGGQLELRALSHILQTPIEIIQADSPPIIVGEEYSKKPLILVYMRHAYGLGEHYNSVTRLVNIVTENCS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Deubiquitinase OTUD6B, DUBA-5, DUBA5, OTU domain-containing protein 6B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-OTUD6B Antibody (A89659)

Western blot analysis of extracts of various cell lines, using Anti-OTUD6B Antibody (A89659) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-OTUD6B Antibody (A89659)

Immunoprecipitation analysis of 200µg extracts of U-87MG cells using 3µg of Anti-OTUD6B Antibody (A89659). This Western blot was performed on the immunoprecipitate using Anti-OTUD6B Antibody (A89659) at a dilution of 1:1000.