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Anti-P Glycoprotein Antibody [ARC0470] (A305706)

Western Blot - Anti-P Glycoprotein Antibody [ARC0470] (A305706) - Antibodies.com Western Blot - Anti-P Glycoprotein Antibody [ARC0470] (A305706) - Antibodies.com Immunohistochemistry - Anti-P Glycoprotein Antibody [ARC0470] (A305706) - Antibodies.com Immunohistochemistry - Anti-P Glycoprotein Antibody [ARC0470] (A305706) - Antibodies.com Immunohistochemistry - Anti-P Glycoprotein Antibody [ARC0470] (A305706) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0470] antibody to P Glycoprotein for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-P Glycoprotein Antibody [ARC0470]
Description
Rabbit monoclonal [ARC0470] antibody to P Glycoprotein.
Applications
WB, IHC
Dilutions
WB: 1:100-1:500, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 393-710 of human P Glycoprotein (P08183).
Sequence
EFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPY
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0470
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
130 - 180 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ABC20, ABCB1, ATP binding cassette, sub family B (MDR/TAP), member 1, ATP-binding cassette sub-family B member 1, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170, MDR1, MDR1_HUMAN, Multidrug resistance 1, Multidrug resistance protein 1, P glycoprotein 1, P gp, P-glycoprotein 1, PGY1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-P Glycoprotein Antibody [ARC0470] (A305706) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-P Glycoprotein Antibody [ARC0470] (A305706)
Western blot analysis of extracts of various cell lines, using Anti-P Glycoprotein Antibody [ARC0470] (A305706) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-P Glycoprotein Antibody [ARC0470] (A305706) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-P Glycoprotein Antibody [ARC0470] (A305706)
Western blot analysis of extracts of Rat liver, using Anti-P Glycoprotein Antibody [ARC0470] (A305706) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunohistochemistry - Anti-P Glycoprotein Antibody [ARC0470] (A305706) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-P Glycoprotein Antibody [ARC0470] (A305706)
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-P Glycoprotein Antibody [ARC0470] (A305706) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-P Glycoprotein Antibody [ARC0470] (A305706) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-P Glycoprotein Antibody [ARC0470] (A305706)
Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-P Glycoprotein Antibody [ARC0470] (A305706) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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