Anti-p21 Antibody [ARC51040] (A307748)

Name: Anti-p21 Antibody [ARC51040]
Brand: Antibodies.com
SKU: A307748
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51040] antibody to p21 for WB and IHC.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-p21 Antibody [ARC51040]

Description

Rabbit monoclonal [ARC51040] antibody to p21.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human CDKN1A/p21CIP1 (NP_000380.1).

Sequence

MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51040

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa / 18 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CAP20, CDK-interacting protein 1, CDKI, CDKN1, Cdkn1a, CDN1A_HUMAN, CIP1, Cyclin Dependent Kinase Inhibitor 1A, Cyclin-dependent kinase inhibitor 1, Cyclin-dependent kinase inhibitor 1A (P21), Cyclin-dependent kinase inhibitor 1A (p21, Cip1), DNA Synthesis Inhibitor, MDA-6, MDA6, Melanoma differentiation-associated protein, Melanoma differentiation-associated protein 6, P21 protein, p21CIP1, p21Cip1/Waf1, p21WAF, PIC1, SDI1, SLC12A9, WAF1, Wild type p53 activated fragment 1, Wild type p53 activated fragment 1 (WAF1), Wildtype p53-activated fragment 1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-p21 Antibody [ARC51040] (A307748)

Western blot analysis of extracts of various cell lines, using Anti-p21 Antibody [ARC51040] (A307748) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Western Blot - Anti-p21 Antibody [ARC51040] (A307748)

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Figure 3: Immunohistochemistry - Anti-p21 Antibody [ARC51040] (A307748)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-p21 Antibody [ARC51040] (A307748) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-p21 Antibody [ARC51040] (A307748)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-p21 Antibody [ARC51040] (A307748) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.