Anti-p27 KIP 1 Antibody [ARC0455] (A305505)

Name: Anti-p27 KIP 1 Antibody [ARC0455]
Brand: Antibodies.com
SKU: A305505

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This product is discontinued

Anti-p27 KIP 1 Antibody [ARC0455] (A305505) has been discontinued and is no longer available.

View all Anti-p27 KIP 1 Antibodies.

Specifications

Name

Anti-p27 KIP 1 Antibody [ARC0455]

Description

Rabbit monoclonal [ARC0455] antibody to p27 KIP 1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 99-198 of human CDKN1B/p27KIP1 (P46527).

Sequence

CKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0455

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

27 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AA408329, AI843786, Cdki1b, CDKN 1B, CDKN 4, CDKN1B, CDKN4, CDN1B_HUMAN, Cyclin Dependent Kinase Inhibitor 1B, Cyclin dependent kinase inhibitor p27, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, Cyclin-dependent kinase inhibitor p27 Kip1, KIP 1, KIP1, MEN1B, MEN4, OTTHUMP00000195098, OTTHUMP00000195099, p27, p27 Kip1, P27-like cyclin-dependent kinase inhibitor, p27Kip1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-p27 KIP 1 Antibody [ARC0455] (A305505)

Western blot analysis of extracts of various cell lines, using Anti-p27 KIP 1 Antibody [ARC0455] (A305505) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-p27 KIP 1 Antibody [ARC0455] (A305505)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-p27 KIP 1 Antibody [ARC0455] (A305505) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-p27 KIP 1 Antibody [ARC0455] (A305505)

Immunohistochemistry analysis of paraffin-embedded human breast tissue using Anti-p27 KIP 1 Antibody [ARC0455] (A305505) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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