Anti-P4HA1 Antibody (A8644)

Name: Anti-P4HA1 Antibody
Brand: Antibodies.com
SKU: A8644
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to P4HA1 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-P4HA1 Antibody

Description

Rabbit polyclonal antibody to P4HA1.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human P4HA1 (NP_001017962.1).

Sequence

STIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKM

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

61 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

2-oxoglutarate-4-dioxygenase subunit alpha-1, 4-hydroxylase, alpha I subunit, 4-PH alpha-1, P4HA, P4HA1_HUMAN, Procollagen-proline, Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I, Prolyl 4-hydroxylase subunit alpha-1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-P4HA1 Antibody (A8644)

Western blot analysis of extracts of various cell lines, using Anti-P4HA1 Antibody (A8644) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Western Blot - Anti-P4HA1 Antibody (A8644)

Western blot analysis of extracts of Rat skeletal muscle, using Anti-P4HA1 Antibody (A8644) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 120.

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Figure 3: Immunofluorescence - Anti-P4HA1 Antibody (A8644)

Immunofluorescence analysis of L929 cells using Anti-P4HA1 Antibody (A8644) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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