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Anti-P4HA3 Antibody (A90971)

Western Blot - Anti-P4HA3 Antibody (A90971) - Antibodies.com Western Blot - Anti-P4HA3 Antibody (A90971) - Antibodies.com Immunofluorescence - Anti-P4HA3 Antibody (A90971) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to P4HA3 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-P4HA3 Antibody
Description
Rabbit polyclonal antibody to P4HA3.
Applications
WB, ICC/IF
Dilutions
WB: 1:1,000-1:5,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-110 of human P4HA3 (NP_878907.1).
Sequence
DPERAAARGDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEAS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
2-oxoglutarate-4-dioxygenase subunit alpha-3, 4-PH alpha-3, P4HA3_HUMAN, Procollagen-proline, Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III, Procollagen-proline, 2-oxoglutarate 4-dioxygenase, alpha subunit, isoform 3, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3, Prolyl 4-hydroxylase subunit alpha-3, Prolyl 4-hydroxylase, alpha polypeptide III, Prolyl 4-hydroxylase, alpha-3 subunit
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-P4HA3 Antibody (A90971) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-P4HA3 Antibody (A90971)
Western blot analysis of extracts of 293T cells, using Anti-P4HA3 Antibody (A90971) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-P4HA3 Antibody (A90971) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-P4HA3 Antibody (A90971)
Western blot analysis of extracts of various cell lines, using Anti-P4HA3 Antibody (A90971) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Immunofluorescence - Anti-P4HA3 Antibody (A90971) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-P4HA3 Antibody (A90971)
Immunofluorescence analysis of NIH/3T3 cells using Anti-P4HA3 Antibody (A90971) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-P4HA3 Antibody (A90971)? Please let us know so that we can list the citation on this page.

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