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Anti-P4HB Antibody (A12685)

Immunofluorescence - Anti-P4HB Antibody (A12685) - Antibodies.com Immunoprecipitation - Anti-P4HB Antibody (A12685) - Antibodies.com
This product is discontinued

Anti-P4HB Antibody (A12685) has been discontinued and is no longer available.

View all P4HB Antibodies.

  • Specifications

Name
Anti-P4HB Antibody
Description
Rabbit polyclonal antibody to P4HB.
Applications
WB, ICC/IF, IP, ELISA
Positive Control
HeLa, 293T, HepG2, NIH/3T3, Mouse liver, Rat liver.
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:20-1:100, IP: 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells
Reactivity
Human, Mouse, Rat
Immunogen
A recombinant fusion protein corresponding to amino acids 329-508 of human P4HB.
Sequence
PESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
57 kDa
Predicted MW
57 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Cellular thyroid hormone-binding protein, ERBA2L, p55, PDI, PDIA1, PO4DB, Prolyl 4-hydroxylase subunit beta, Protein disulfide-isomerase
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Immunofluorescence - Anti-P4HB Antibody (A12685) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-P4HB Antibody (A12685)

P4HB expression in U2OS cells analyzed by immunofluorescence. Staining was performed with Anti-P4HB Antibody (A12685) at a dilution of 1:400. Nuclei were stained with DAPI (blue).
Immunoprecipitation - Anti-P4HB Antibody (A12685) - Antibodies.comEnlarge Image

Immunoprecipitation - Anti-P4HB Antibody (A12685)

P4HB expression in 200 µg extracts of SW620 cells analyzed by western blot following immunoprecipitation, both using Anti-P4HB Antibody (A12685) at 3 µg and a dilution of 1:500, respectively.

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Proteins predicted to interact with P4HB

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.6% Relevancy Score
99.6% Relevancy Score
99.6% Relevancy Score
99.4% Relevancy Score
99.3% Relevancy Score
98.3% Relevancy Score
98% Relevancy Score
96.6% Relevancy Score