Anti-p53 Antibody (A295374)

Name: Anti-p53 Antibody
Brand: Antibodies.com
SKU: A295374
Price: 475.0 USD
Availability: InStock

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$475

Rabbit polyclonal antibody to p53 for WB, IP, IHC, ChIP-Seq and Flow Cytometry.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

ER Marker Mitochondrial Marker

Specifications

Name

Anti-p53 Antibody

Description

Rabbit polyclonal antibody to p53.

Applications

WB, IP, IHC, ChIP-Seq, Flow Cytometry

Dilutions

ChIP-Seq: 4 µg / 30 µg chromatin, Flow Cytometry: 1.5 µg per 1 x 10⁶ cells in a 150 µl volume, IHC: 1:1,000-1:5,000, IP: 2-5µg / mg lysate, WB: 1:2,000-1:10,000

Reactivity

Human

Immunogen

Synthetic peptide within amino acids 50-100 of human p53.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonyms

Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Anti-p53 Antibody (A295374)

Chromatin from MCF-7 cells was immunoprecipitated with Anti-p53 Antibody (A295374) and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using Anti-p53 Antibody (A295374). ChIP-seq validation performed by Active Motif, Carlsbad, CA.

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Figure 2: Flow Cytometry - Anti-p53 Antibody (A295374)

Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 X 10^6 cells were stained with 1.5 µg anti-KLH IgG control or Anti-p53 Antibody (A295374) and secondary FITC-conjugated goat anti-rabbit (in a 150µl reaction). anti-KLH control IgG (black); anti-p53 (red).

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Figure 3: Immunohistochemistry - Anti-p53 Antibody (A295374)

Sample: FFPE section of human lung cancer Antibody: Anti-p53 Antibody (A295374) was used at 1:5,000 (0.2 µg/ml). Detection: DAB.

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Figure 4: Immunoprecipitation - Anti-p53 Antibody (A295374)

Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Anti-p53 Antibody (A295374) was used for IP at 3 µg per reaction. p53 was also immunoprecipitated using another rabbit anti-P53 antibody. For blotting immunoprecipitated p53, Anti-p53 Antibody (A295374) was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.