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Anti-p75 NGF Receptor Antibody [ARC0284] (A306428)

Western Blot - Anti-p75 NGF Receptor Antibody [ARC0284] (A306428) - Antibodies.com Western Blot - Anti-p75 NGF Receptor Antibody [ARC0284] (A306428) - Antibodies.com Immunohistochemistry - Anti-p75 NGF Receptor Antibody [ARC0284] (A306428) - Antibodies.com
$470
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Rabbit monoclonal [ARC0284] antibody to p75 NGF Receptor for WB and IHC.

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Anti-p75 NGF Receptor Antibody
Rabbit polyclonal antibody to p75 NGF Receptor for CM, ELISA, ICC, IF, IHC, IP and WB.
Name
Anti-p75 NGF Receptor Antibody [ARC0284]
Description
Rabbit monoclonal [ARC0284] antibody to p75 NGF Receptor.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human p75 NGF Receptor (P08138).
Sequence
KLHSDSGISVDSQSLHDQQPHTQTASGQALKGDGGLYSSLPPAKREEVEKLLNGSAGDTWRHLAGELGYQPEHIDSFTHEACPVRALLASWATQDSATLDA
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0284
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
75 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CD271, CD271 antigen, Gp80 LNGFR, Gp80-LNGFR, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Nerve growth factor receptor, Nerve growth factor receptor TNFR superfamily member 16, NGF receptor, Ngfr, p75 ICD, p75 Neurotrophin receptor, p75 NTR, p75(NTR), p75NTR, TNFR Superfamily Member 16, TNFRSF16, TNR16_HUMAN, Tumor necrosis factor receptor superfamily member 16
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-p75 NGF Receptor Antibody [ARC0284] (A306428) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-p75 NGF Receptor Antibody [ARC0284] (A306428)
Western blot analysis of extracts of various cell lines, using Anti-p75 NGF Receptor Antibody [ARC0284] (A306428) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-p75 NGF Receptor Antibody [ARC0284] (A306428) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-p75 NGF Receptor Antibody [ARC0284] (A306428)
Western blot analysis of extracts of various cell lines, using Anti-p75 NGF Receptor Antibody [ARC0284] (A306428) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry - Anti-p75 NGF Receptor Antibody [ARC0284] (A306428) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-p75 NGF Receptor Antibody [ARC0284] (A306428)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-p75 NGF Receptor Antibody [ARC0284] (A306428) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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