Anti-PACAP Antibody (A88543)

Name: Anti-PACAP Antibody
Brand: Antibodies.com
SKU: A88543
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to PACAP for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PACAP Antibody

Description

Rabbit polyclonal antibody to PACAP.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human MZB1 (NP_057543.2).

Sequence

MRLSLPLLLLLLGAWAIPGGLGDRAPLTATAPQLDDEEMYSAHMPAHLRCDACRAVAYQMWQNLAKAETKLHTSNSGGRRELSELVYTDVLDRSCSRNWQDYGVREVDQVKRLTGPGLSEGPEPSISVMVTGGPWPTRLSRTCLHYLGEFGEDQIYEAHQQGRGALEALLCGGPQGACSEKVSATREEL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

21 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

adenylate cyclase activating polypeptide 1, adenylate cyclase activating polypeptide 1 (pituitary), PACAP-related peptide, Pituitary adenylate cyclase-activating polypeptide, Pituitary adenylate cyclase-activating polypeptide 27, Pituitary adenylate cyclase-activating polypeptide 38, prepro-PACAP, PRP-48

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-PACAP Antibody (A88543)

Western blot analysis of extracts of Raji cells, using Anti-PACAP Antibody (A88543) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-PACAP Antibody (A88543)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-PACAP Antibody (A88543) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PACAP Antibody (A88543)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PACAP Antibody (A88543) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PACAP Antibody (A88543)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-PACAP Antibody (A88543) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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