Anti-PAH Antibody [ARC2936] (A307869)

Name: Anti-PAH Antibody [ARC2936]
Brand: Antibodies.com
SKU: A307869

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This product is discontinued

Anti-PAH Antibody [ARC2936] (A307869) has been discontinued and is no longer available.

View all Anti-PAH Antibodies.

Specifications

Name

Anti-PAH Antibody [ARC2936]

Description

Rabbit monoclonal [ARC2936] antibody to PAH.

Applications

WB, ICC/IF

Dilutions

WB: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PAH (P00439).

Sequence

MSTAVLENPGLGRKLSDFGQETSYIEDNCNQNGAISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRLKKDEYEFFTHLDKRSLPALTNIIKILRH

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2936

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

PH, PH4H_HUMAN, Phe 4 monooxygenase, Phe-4-monooxygenase, Phenylalanine 4 hydroxylase, Phenylalanine hydroxylase, Phenylalanine-4-hydroxylase, PKU, PKU1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PAH Antibody [ARC2936] (A307869)

Western blot analysis of extracts of various cell lines, using Anti-PAH Antibody [ARC2936] (A307869) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-PAH Antibody [ARC2936] (A307869)

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Figure 3: Immunofluorescence - Anti-PAH Antibody [ARC2936] (A307869)

Immunofluorescence analysis of HepG2 cells using Anti-PAH Antibody [ARC2936] (A307869) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-PAH Antibody [ARC2936] (A307869)

Immunofluorescence analysis of mouse liver cells using Anti-PAH Antibody [ARC2936] (A307869) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).