Figure 2: Western Blot - Anti-pan Histone H3 Antibody [RM188] (A121319)
Western blot of recombinant Histone H3.3 and Histone H3.1 proteins and A375, HEK293, HeLa, and SK-MEL-2 whole cell lysates, using Anti-pan Histone H3 Antibody [RM188] at 0.025µg/ml, showed a band of recombinant H3.1 and H3.3 proteins, and total Histone H3 in A375, HEK293, HeLa, and SK-MEL-2 cells.
Immunocytochemistry of HeLa cells, using Anti-pan Histone H3 Antibody [RM188] (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Sandwich ELISA against acetylated Histone H3 at Lys9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. 1µg/ml of Anti-pan Histone H3 Antibody [RM188] was used as the capture antibody and 1µg/ml of Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (Biotin) was used as the detection antibody.
Sandwich ELISA against acetylated Histone H3 at Lys79 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. 5µg/ml of Anti-Histone H3 (acetyl Lys79) Antibody [RM156] was used as the capture antibody and 1µg/ml of Anti-pan Histone H3 Antibody [RM188] (Biotin) was used as the detection antibody.
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