Anti-PAR4 Antibody [ARC1980] (A309260)

Name: Anti-PAR4 Antibody [ARC1980]
Brand: Antibodies.com
SKU: A309260
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1980] antibody to PAR4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-PAR4 Antibody [ARC1980]

Description

Rabbit monoclonal [ARC1980] antibody to PAR4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 241-340 of human PAR4 (Q96IZ0).

Sequence

YSRTDRSGFPRYNRDANVSGTLVSSSTLEKKIEDLEKEVVRERQENLRLVRLMQDKEEMIGKLKEEIDLLNRDLDDIEDENEQLKQENKTLLKVVGQLTR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1980

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

41 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

2310001G03Rik, PAR 4, PAR-4, Pawr, PAWR_HUMAN, PRKC Apoptosis WT1 Regulator, PRKC apoptosis WT1 regulator protein, Prostate apoptosis response 4 protein, Prostate apoptosis response protein 4, prostate apoptosis response protein PAR-4, Transcriptional repressor Par-4-like protein PAWR, Transcriptional repressor PAR4, WT1 Interacting Protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-PAR4 Antibody [ARC1980] (A309260)

Western blot analysis of extracts of various cell lines, using Anti-PAR4 Antibody [ARC1980] (A309260) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-PAR4 Antibody [ARC1980] (A309260)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PAR4 Antibody [ARC1980] (A309260) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-PAR4 Antibody [ARC1980] (A309260)

Immunofluorescence analysis of HeLa cells using Anti-PAR4 Antibody [ARC1980] (A309260) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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