Anti-PARP1 Antibody [AMC0216] (A306481)

Name: Anti-PARP1 Antibody [AMC0216]
Brand: Antibodies.com
SKU: A306481

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This product is discontinued

Anti-PARP1 Antibody [AMC0216] (A306481) has been discontinued and is no longer available.

View all Anti-PARP1 Antibodies.

Specifications

Name

Anti-PARP1 Antibody [AMC0216]

Description

Mouse monoclonal [AMC0216] antibody to PARP1.

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 662-1014 of human PARP1 (NP_001609.2).

Sequence

KSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW

Host

Mouse

Clonality

Monoclonal

Clone ID

AMC0216

Isotype

IgG2a

Light Chains

kappa

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

89 kDa / 130 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADP ribosyltransferase, ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase), ADP ribosyltransferase diphtheria toxin like 1, ADP ribosyltransferase NAD(+), ADPRT, ADPRT 1, ADPRT1, ARTD1, msPARP, NAD(+) ADP ribosyltransferase 1, NAD(+) ADP-ribosyltransferase 1, pADPRT 1, pADPRT1, PARP, PARP 1, PARP-1, PARP1_HUMAN, Poly (ADP ribose) polymerase 1, poly (ADP ribose) polymerase family, member 1, Poly [ADP-ribose] polymerase 1, Poly(ADP ribose) polymerase, poly(ADP ribose) synthetase, poly(ADP ribosyl)transferase, Poly[ADP ribose] synthetase 1, Poly[ADP-ribose] synthase 1, PPOL

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (1)

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Figure 1: Western Blot - Anti-PARP1 Antibody [AMC0216] (A306481)

Western blot analysis of extracts of various cell lines, using Anti-PARP1 Antibody [AMC0216] (A306481) at 1:1,000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 1s.

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