Rabbit polyclonal antibody to Paxillin (Phospho-Ser272)
Specificity
The Antibody detects endogenous levels of Paxillin only when phosphorylated at Ser272
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of Serine 272(M-A-S(p)-L-S) derived from Human Paxillin.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
FLJ16691, FLJ23042, Paired box protein Pax 1, PAX 1, PAX1, Paxillin alpha, PAXI_HUMAN, PXN, PXN protein
Western blot analysis of extracts from HeLa and JK using Paxillin (Phospho-Ser272) Antibody #11762.The lane on the right is treated with the antigen-specific peptide.
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