Anti-PBX1 Antibody [PCRP-PBX1-3C8] - BSA and Azide free (A278320)

Name: Anti-PBX1 Antibody [PCRP-PBX1-3C8] - BSA and Azide free
Brand: Antibodies.com
SKU: A278320
Price: 525.0 USD
Availability: InStock

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$525

Mouse monoclonal [PCRP-PBX1-3C8] antibody to PBX1 for ELISA, IP, Flow Cytometry and IF.

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Alternative Formats and ConjugatesAlternative Formats

Unconjugated

Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

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Tags:

Monospecific Antibody

Specifications

Name

Anti-PBX1 Antibody [PCRP-PBX1-3C8] - BSA and Azide free

Description

Mouse monoclonal [PCRP-PBX1-3C8] antibody to PBX1.

Specificity

Pbx 1, 2, 3 and 4 are members of the TALE (three amino acid loop extension) family of homeodomain-containing proteins. Human pre-B cell acute leukemias are frequently associated with a t(1;19)(q23;p13.3) chromosomal rearrangement, which creates a chimeric gene encoding a fusion between the E2A and Pbx 1 gene products. Pbx 2 and Pbx 3 share 92% and 94% respective identities with Pbx 1 over a 266 amino acid region flanking their homeobox domains, while all three proteins are quite divergent at their amino- and carboxy-termini. Two forms of Pbx 1 and Pbx 3 each differ primarily in their carboxy-termini and result from alternative mRNA splicing. Unlike other homeotic selector genes which are expressed transiently during development and differentiation, Pbx gene transcripts are ubiquitously expressed in both fetal and adult tissues and cell lines. Additionally, Pbx 2 and Pbx 3 transcripts are detected in lymphoid cells, which do not express Pbx 1. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase.

Applications

ELISA, IP, Flow Cytometry, IF

Dilutions

IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Reactivity

Human

Immunogen

Recombinant full-length human PBX1 protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

PCRP-PBX1-3C8

Isotype

IgG2b

Conjugate

Unconjugated

Purification

Protein A/G chromatography.

Concentration

1 mg/ml

Molecular Weight

86.1 kDa

Product Form

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-PBX1 Antibody [PCRP-PBX1-3C8] (A277732).

Synonyms

DKFZp686B09108, Homeo box protein PBX1, Homeo box protein PRL, Homeobox protein PBX 1, Homeobox protein PBX1, Homeobox protein PRL, MGC126627, PBX 1, PBX1_HUMAN, Pre B cell leukemia homeobox 1, Pre B cell leukemia transcription factor 1, Pre-B-cell leukemia transcription factor 1, PRL

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: SDS-PAGE - Anti-PBX1 Antibody [PCRP-PBX1-3C8] - BSA and Azide free (A278320)

SDS-PAGE analysis of Anti-PBX1 Antibody [PCRP-PBX1-3C8] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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Figure 2: Flow Cytometry - Anti-PBX1 Antibody [PCRP-PBX1-3C8] - BSA and Azide free (A278320)

Flow cytometric analysis of PFA-fixed HeLa cells using Anti-PBX1 Antibody [PCRP-PBX1-3C8] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Isotype Control (Red).

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Figure 3: Immunofluorescence - Anti-PBX1 Antibody [PCRP-PBX1-3C8] - BSA and Azide free (A278320)

Immunofluorescent analysis of PFA-fixed HeLa cells stained with Anti-PBX1 Antibody [PCRP-PBX1-3C8] followed by Goat Anti-Mouse IgG (CF® 488) (Green). CF® 640A Phalloidin (Red).

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Figure 4: Anti-PBX1 Antibody [PCRP-PBX1-3C8] - BSA and Azide free (A278320)

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-PBX1 Antibody [PCRP-PBX1-3C8]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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