Anti-PCK2 Antibody [ARC1017] (A305761)

Name: Anti-PCK2 Antibody [ARC1017]
Brand: Antibodies.com
SKU: A305761

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This product is discontinued

Anti-PCK2 Antibody [ARC1017] (A305761) has been discontinued and is no longer available.

View all Anti-PCK2 Antibodies.

Specifications

Name

Anti-PCK2 Antibody [ARC1017]

Description

Rabbit monoclonal [ARC1017] antibody to PCK2.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 550-640 of human PEPCK/PEPCK/PCK2 (Q16822).

Sequence

ENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1017

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

71 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EC 4.1.1.32, GTP mitochondrial precursor, HGNC:8725, mitochondrial, Mitochondrial phosphoenolpyruvate carboxykinase 2, OTTHUMP00000164700, PCKGM_HUMAN, PE, PEP carboxykinase, PEPCK, PEPCK deficiency mitochondrial, PEPCK M, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase 2 (mitochondrial), Phosphoenolpyruvate carboxykinase 2 mitochondrial, Phosphoenolpyruvate carboxykinase [GTP], Phosphoenolpyruvate carboxylase, Phosphopyruvate carboxylase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-PCK2 Antibody [ARC1017] (A305761)

Western blot analysis of extracts of various cell lines, using Anti-PCK2 Antibody [ARC1017] (A305761) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-PCK2 Antibody [ARC1017] (A305761)

Western blot analysis of extracts from wild type(WT) and PEPCK/PCK2 knockout (KO) 293T cells, using Anti-PCK2 Antibody [ARC1017] (A305761) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunofluorescence - Anti-PCK2 Antibody [ARC1017] (A305761)

Immunofluorescence analysis of HeLa cells using Anti-PCK2 Antibody [ARC1017] (A305761) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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