Anti-PDCD4 Antibody (A11542)

Name: Anti-PDCD4 Antibody
Brand: Antibodies.com
SKU: A11542
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to PDCD4 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-PDCD4 Antibody

Description

Rabbit polyclonal antibody to PDCD4.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PDCD4 (NP_055271.2).

Sequence

MDVENEQILNVNPADPDNLSDSLFSGDEENAGTEEIKNEINGNWISASSINEARINAKAKRRLRKNSSRDSGRGDSVSDSGSDALRSGLTVPTSPKGRLLDRRSRSGKGRGLPKKGGAGGKGVWGTPGQVYDVEEVDVKDPNYDDDQENCVYETVVLPLDERAFEKTLTPIIQEYFEHGDTNEVAEMLRDLNLGEMKSGVPVLAVSLALEGKASHREMTSKLLSDLCGTVMSTTDVEKSFDKLLKDLPELALDTPRAPQL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Death up-regulated gene protein, Dug, H731, Ma3, MGC33046, MGC33047, Neoplastic transformation inhibitor, Neoplastic transformation inhibitor protein, Nuclear antigen H731, Nuclear antigen H731 like, Nuclear antigen H731 like protein, Nuclear antigen H731-like, PDCD 4, PDCD4_HUMAN, Programmed cell death 4, programmed cell death 4 (neoplastic transformation inhibitor), Programmed cell death protein 4, Protein 197/15a, Protein MA-3, Tis, Topoisomerase-inhibitor suppressed protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-PDCD4 Antibody (A11542)

Western blot analysis of extracts of various cell lines, using Anti-PDCD4 Antibody (A11542) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Western Blot - Anti-PDCD4 Antibody (A11542)

Western blot analysis of extracts from normal (control) and PDCD4 knockout (KO) 293T cells, using Anti-PDCD4 Antibody (A11542) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-PDCD4 Antibody (A11542)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-PDCD4 Antibody (A11542) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PDCD4 Antibody (A11542)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-PDCD4 Antibody (A11542) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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