Anti-PDGF B Antibody [ARC53712] (A308519)

Name: Anti-PDGF B Antibody [ARC53712]
Brand: Antibodies.com
SKU: A308519

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This product is discontinued

Anti-PDGF B Antibody [ARC53712] (A308519) has been discontinued and is no longer available.

View all Anti-PDGF B Antibodies.

Specifications

Name

Anti-PDGF B Antibody [ARC53712]

Description

Rabbit monoclonal [ARC53712] antibody to PDGF B.

Applications

WB, IHC

Dilutions

WB: 1:2,000-1:20,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 142-241 of human PDGFB (NP_002599.1).

Sequence

RPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVTRSPGGSQEQRAKTPQTRVTIRTVRVRRPPKGKHRKFKHTHDKTALKETLGA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53712

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Becaplermin, c sis, FLJ12858, Oncogene SIS, PDGF 2, PDGF B chain, PDGF Beta, PDGF subunit B, PDGF-2, PDGF2, Pdgfb, PDGFB_HUMAN, Platelet derived growth factor 2, Platelet derived growth factor B chain, Platelet derived growth factor beta, Platelet derived growth factor beta polypeptide, Platelet derived growth factor beta polypeptide (oncogene SIS), Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog), Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Platelet-derived growth factor subunit B, Proto-oncogene c-Sis, Simian sarcoma viral (v sis) oncogene homolog, SIS, SSV, v sis platelet derived growth factor beta polypeptide

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-PDGF B Antibody [ARC53712] (A308519)

Western blot analysis of extracts of various cell lines, using Anti-PDGF B Antibody [ARC53712] (A308519) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-PDGF B Antibody [ARC53712] (A308519)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-PDGF B Antibody [ARC53712] (A308519) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PDGF B Antibody [ARC53712] (A308519)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-PDGF B Antibody [ARC53712] (A308519) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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