Anti-PEA15 Antibody [4D2] (A270553)

Immunofluorescent analysis of rat brain section stained using Anti-PEA15 Antibody [4D2] (A270553), at a dilution of 1:1,000, in red. The tissue was co-stained using Anti-GFAP Antibody (A85419), at a dilution of 1:5,000, in green. Nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion of the rat with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained using the above antibodies. The Anti-PEA15 Antibody [4D2] (A270553) labels the cytoplasm of certain cells which are not labelled by the astrocyte specific Anti-GFAP Antibody (A85419).

Western blot analysis of different tissue lysates using Anti-PEA15 Antibody [4D2] (A270553), at a dilution of 1:1,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] rat whole brain, [3] rat cerebellum, [4] mouse whole brain, [5] cow cortex and [6] cow cerebellum. The single strong band at about 15 kDa corresponds to the PEA-15 protein.