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Anti-Pericentrin Antibody (A305977)

Western Blot - Anti-Pericentrin Antibody (A305977) - Antibodies.com Immunofluorescence - Anti-Pericentrin Antibody (A305977) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Pericentrin for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Pericentrin Antibody
Description
Rabbit polyclonal antibody to Pericentrin.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 95-212 of human PCNT (NP_006022.3).
Sequence
GEKREDLEQLQQKQVNDHPPEQCGMFTVSDHPPEQHGMFTVGDHPPEQRGMFTVSDHPPEQHGMFTVSDHPPEQRGMFTISDHQPEQRGMFTVSDHTPEQRGIFTISDHPAEQRGMFT
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
251 kDa / 378 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Pericentrin Antibody (A305977) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Pericentrin Antibody (A305977)
Western blot analysis of extracts of various cell lines, using Anti-Pericentrin Antibody (A305977) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence - Anti-Pericentrin Antibody (A305977) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Pericentrin Antibody (A305977)
Immunofluorescence analysis of U-2 OS cells using Anti-Pericentrin Antibody (A305977) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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