Anti-PFKFB3 Antibody [ARC50572] (A306539)

Name: Anti-PFKFB3 Antibody [ARC50572]
Brand: Antibodies.com
SKU: A306539

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This product is discontinued

Anti-PFKFB3 Antibody [ARC50572] (A306539) has been discontinued and is no longer available.

View all Anti-PFKFB3 Antibodies.

Specifications

Name

Anti-PFKFB3 Antibody [ARC50572]

Description

Rabbit monoclonal [ARC50572] antibody to PFKFB3.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human PFKFB3 (NP_004557.1).

Sequence

LDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50572

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase, 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3, 6-bisphosphatase, 6-P2ase 3, 6-P2ASE brain/placenta-type isozyme, 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme, 6PF 2-K/Fru 2,6 P2ase 3, 6PF-2-K/Fru-2, F263_HUMAN, fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase, Fructose-2, Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase, iPFK 2, iPFK-2, IPFK2, PFK/FBPase 3, PFK2, Renal carcinoma antigen NY REN 56, Renal carcinoma antigen NY-REN-56, uPFK 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PFKFB3 Antibody [ARC50572] (A306539)

Western blot analysis of HeLa, using Anti-PFKFB3 Antibody [ARC50572] (A306539) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-PFKFB3 Antibody [ARC50572] (A306539)

Western blot analysis of Mouse brain, using Anti-PFKFB3 Antibody [ARC50572] (A306539) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Figure 3: Immunohistochemistry - Anti-PFKFB3 Antibody [ARC50572] (A306539)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-PFKFB3 Antibody [ARC50572] (A306539) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PFKFB3 Antibody [ARC50572] (A306539)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-PFKFB3 Antibody [ARC50572] (A306539) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.