Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Bcl-XL-binding protein v68, BXLBv68, MGC5352, mitochondrial, PGAM5_HUMAN, Phosphoglycerate mutase family member 5, Serine/threonine protein phosphatase PGAM5 mitochondrial, Serine/threonine-protein phosphatase PGAM5
Figure 1: Western Blot - Anti-PGAM5 Antibody (A89258)
Western blot analysis of various lysates, using Anti-PGAM5 Antibody (A89258) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PGAM5 Antibody (A89258) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-PGAM5 Antibody (A89258) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-PGAM5 Antibody (A89258) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of MCF7 cells using Anti-PGAM5 Antibody (A89258) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).
