Anti-PGP9.5 Antibody [ARC50379] (A307671)

Name: Anti-PGP9.5 Antibody [ARC50379]
Brand: Antibodies.com
SKU: A307671

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This product is discontinued

Anti-PGP9.5 Antibody [ARC50379] (A307671) has been discontinued and is no longer available.

View all Anti-PGP9.5 Antibodies.

Specifications

Name

Anti-PGP9.5 Antibody [ARC50379]

Description

Rabbit monoclonal [ARC50379] antibody to PGP9.5.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:10,000-1:40,000, IHC: 1:50-1:200, ICC/IF: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PGP95/UCHL1 (NP_004172.2).

Sequence

MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALC

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50379

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Epididymis luminal protein 117, Epididymis secretory protein Li 53, HEL 117, HEL S 53, NDGOA, Neuron cytoplasmic protein 9.5, OTTHUMP00000218137, OTTHUMP00000218139, OTTHUMP00000218140, OTTHUMP00000218141, Park 5, PARK5, PGP 9.5, PGP95, Protein gene product 9.5, Ubiquitin C terminal esterase L1, Ubiquitin C terminal hydrolase, Ubiquitin C terminal hydrolase L1, Ubiquitin carboxyl terminal esterase L1, Ubiquitin carboxyl terminal hydrolase isozyme L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L1, Ubiquitin thiolesterase, Ubiquitin thiolesterase L1, UCH-L1, UCHL1, UCHL1_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-PGP9.5 Antibody [ARC50379] (A307671)

Western blot analysis of various lysates, using Anti-PGP9.5 Antibody [ARC50379] (A307671) at 1:40,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-PGP9.5 Antibody [ARC50379] (A307671)

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-PGP9.5 Antibody [ARC50379] (A307671) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-PGP9.5 Antibody [ARC50379] (A307671)

Immunohistochemistry analysis of paraffin-embedded mouse pancreas using Anti-PGP9.5 Antibody [ARC50379] (A307671) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PGP9.5 Antibody [ARC50379] (A307671)

Immunohistochemistry analysis of paraffin-embedded rat colon using Anti-PGP9.5 Antibody [ARC50379] (A307671) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.