Anti-PHD3 Antibody [ARC52452] (A307618)

Name: Anti-PHD3 Antibody [ARC52452]
Brand: Antibodies.com
SKU: A307618

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This product is discontinued

Anti-PHD3 Antibody [ARC52452] (A307618) has been discontinued and is no longer available.

View all Anti-PHD3 Antibodies.

Specifications

Name

Anti-PHD3 Antibody [ARC52452]

Description

Rabbit monoclonal [ARC52452] antibody to PHD3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:2,000-1:20,000, IHC: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD3 (NP_071356.1).

Sequence

MPLGHIMRLDLEKIALEYIVPCLHEVGFCYLDNFLGEVVGDCVLERVKQLHCTGALRDGQLAGPRAGVSKRHLRGDQITWIGGNEEGCEAISFLLSLIDR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC52452

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

32 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Egl 9 family hypoxia inducible factor 3, Egl nine homolog 3, Egl nine homolog 3 (C. elegans), Egl nine like protein 3 isoform, EGL9 homolog of C. elegans 3, EGLN3, EGLN3_HUMAN, Factor responsive smooth muscle protein, HIF Prolyl Hydroxylase 3, HIF-PH3, HIF-prolyl hydroxylase 3, HIFP4H3, HIFPH3, HPH-1, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 3, P4H3, Prolyl Hydroxylase Domain Containing Protein 3, Prolyl hydroxylase domain-containing protein 3, SM20

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-PHD3 Antibody [ARC52452] (A307618)

Western blot analysis of extracts of various cell lines, using Anti-PHD3 Antibody [ARC52452] (A307618) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-PHD3 Antibody [ARC52452] (A307618)

Western blot analysis of extracts of BxPC-3 cells, using Anti-PHD3 Antibody [ARC52452] (A307618) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-PHD3 Antibody [ARC52452] (A307618)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PHD3 Antibody [ARC52452] (A307618) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PHD3 Antibody [ARC52452] (A307618)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-PHD3 Antibody [ARC52452] (A307618) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.