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Anti-PHF8 Antibody [ARC2306] (A309000)

Western Blot - Anti-PHF8 Antibody [ARC2306] (A309000) - Antibodies.com Western Blot - Anti-PHF8 Antibody [ARC2306] (A309000) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2306] antibody to PHF8 for WB.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-PHF8 Antibody [ARC2306]
Description
Rabbit monoclonal [ARC2306] antibody to PHF8.
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 800-900 of human PHF8 (Q9UPP1).
Sequence
TVSNSPASQRTPGKRPIKRPAYWRTESEEEEENASLDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGT
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2306
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
140 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Histone lysine demethylase PHF8, JHDM1F, Jumonji C domain containing histone demethylase 1F, MRXSSD, PHD finger protein 8, PHF8_HUMAN, ZNF422
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-PHF8 Antibody [ARC2306] (A309000) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-PHF8 Antibody [ARC2306] (A309000)
Western blot analysis of extracts of HeLa cells, using Anti-PHF8 Antibody [ARC2306] (A309000) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-PHF8 Antibody [ARC2306] (A309000) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-PHF8 Antibody [ARC2306] (A309000)
Western blot analysis of extracts of various cell lines, using Anti-PHF8 Antibody [ARC2306] (A309000) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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