Western Blot - Anti-Phospholipaselipase C beta 3/PLCB3 (phospho Ser1105) Antibody (A88325)
Western blot analysis of extracts of HeLa cells, using Anti-Phospholipaselipase C beta 3/PLCB3 (phospho Ser1105) Antibody (A88325) at 1:1,000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 10s.
Publishing research using Anti-Phospholipaselipase C beta 3/PLCB3 (phospho Ser1105) Antibody (A88325)? Please let us know so that we can list the citation on this page.