Immunocytochemistry/Immunofluorescence analysis of human HeLa cells, fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Phosphotyrosine Antibody (A304808), at 1:50 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 120 min at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at room temperature. Localization: Nucleus. Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite.
Immunocytochemistry/Immunofluorescence analysis of human HeLa cells, fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Phosphotyrosine Antibody (A304808), at 1:50 for 12 hours at 4°C. The secondary antibody used was APC Goat Anti-Rabbit (red) at 1:200 for 120 min at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at room temperature. Localization: Nucleus. Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Phosphotyrosine Antibody. (C) Composite.
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